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ythdc1 expression vectors  (Genecopoeia)


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    Structured Review

    Genecopoeia ythdc1 expression vectors
    Primers used in this study.
    Ythdc1 Expression Vectors, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ythdc1 expression vectors/product/Genecopoeia
    Average 94 stars, based on 2 article reviews
    ythdc1 expression vectors - by Bioz Stars, 2026-03
    94/100 stars

    Images

    1) Product Images from "Multiple Phosphorylations of SR Protein SRSF3 and Its Binding to m 6 A Reader YTHDC1 in Human Cells"

    Article Title: Multiple Phosphorylations of SR Protein SRSF3 and Its Binding to m 6 A Reader YTHDC1 in Human Cells

    Journal: Cells

    doi: 10.3390/cells11091461

    Primers used in this study.
    Figure Legend Snippet: Primers used in this study.

    Techniques Used: Sequencing

    Interaction validation of YTHDC1 with SRSF3. ( a ) YTHDC1 and its fragment structure indicating the position of glutamic acid-rich region (Glu-rich), YT521-B homology (YTH) domain and arginine-rich region (Arg-rich). ( b ) HeLa cells were co-transfected with GFP-SRSF3, Myc-YTHDC1, or their mutants. The lysates of co-transfected cells were immunoprecipitated using anti-Myc antibody. Myc-YTHDC1 interaction with GFP-SRSF3 and its fragments was detected by western blotting using anti-GFP or anti-Myc antibodies (( b ), left ); the result of phos-tag gel is also shown in (( b ), right ). Some nonspecific bands are observed.
    Figure Legend Snippet: Interaction validation of YTHDC1 with SRSF3. ( a ) YTHDC1 and its fragment structure indicating the position of glutamic acid-rich region (Glu-rich), YT521-B homology (YTH) domain and arginine-rich region (Arg-rich). ( b ) HeLa cells were co-transfected with GFP-SRSF3, Myc-YTHDC1, or their mutants. The lysates of co-transfected cells were immunoprecipitated using anti-Myc antibody. Myc-YTHDC1 interaction with GFP-SRSF3 and its fragments was detected by western blotting using anti-GFP or anti-Myc antibodies (( b ), left ); the result of phos-tag gel is also shown in (( b ), right ). Some nonspecific bands are observed.

    Techniques Used: Biomarker Discovery, Transfection, Immunoprecipitation, Western Blot

    Interaction of YTHDC1 fragment with SRSF3. ( a ) YTHDC1 and its 493–727 fragment structure. ( b ) HeLa cells were co-transfected with GFP-SRSF3 wild type and mutants, and Myc-YTHDC1(493–727). The interaction between the C-terminal region of YTHDC1 with GFP-SRSF3 and its fragments was detected by western blotting using anti-GFP or anti-Myc antibodies. Some nonspecific bands are observed.
    Figure Legend Snippet: Interaction of YTHDC1 fragment with SRSF3. ( a ) YTHDC1 and its 493–727 fragment structure. ( b ) HeLa cells were co-transfected with GFP-SRSF3 wild type and mutants, and Myc-YTHDC1(493–727). The interaction between the C-terminal region of YTHDC1 with GFP-SRSF3 and its fragments was detected by western blotting using anti-GFP or anti-Myc antibodies. Some nonspecific bands are observed.

    Techniques Used: Transfection, Western Blot

    ( a ) HeLa cells were co-transfected with Myc-YTHDC1, GFP-SRSF3, or GFP-SRSF3 deletion mutants. The lysates of co-transfected cells were immunoprecipitated using anti-Myc antibodies. Lysates and IP samples were detected by western blotting using anti-GFP or anti-Myc antibodies. Phosphorylation status of immunoprecipitated SRSF3 and mutants was analyzed by separation using phos-tag gel (bottom panel). ( b ) YTHDC1 and its 1–353 fragment structure. ( c ) HeLa cells were co-transfected with GFP-YTHDC1(1–353), Myc-SRSF3, or Myc-SRSF3 deletion mutants. Lysates of co-transfected cells were immunoprecipitated using anti-GFP antibody. Lysates and IP samples were detected using anti-GFP or anti-Myc antibodies. YTHDC1(1–353) shows a clear interaction with SRSF3(1–132).
    Figure Legend Snippet: ( a ) HeLa cells were co-transfected with Myc-YTHDC1, GFP-SRSF3, or GFP-SRSF3 deletion mutants. The lysates of co-transfected cells were immunoprecipitated using anti-Myc antibodies. Lysates and IP samples were detected by western blotting using anti-GFP or anti-Myc antibodies. Phosphorylation status of immunoprecipitated SRSF3 and mutants was analyzed by separation using phos-tag gel (bottom panel). ( b ) YTHDC1 and its 1–353 fragment structure. ( c ) HeLa cells were co-transfected with GFP-YTHDC1(1–353), Myc-SRSF3, or Myc-SRSF3 deletion mutants. Lysates of co-transfected cells were immunoprecipitated using anti-GFP antibody. Lysates and IP samples were detected using anti-GFP or anti-Myc antibodies. YTHDC1(1–353) shows a clear interaction with SRSF3(1–132).

    Techniques Used: Transfection, Immunoprecipitation, Western Blot, Phospho-proteomics

    HeLa cells were co-transfected with GFP-YTHDC1(1–353), Myc-SRSF3, or Myc-SRSF3 deletion mutants. The lysates of co-transfected cells were immunoprecipitated using anti-GFP antibody. Lysates and IP samples were detected by western blotting using anti-GFP or anti-Myc antibodies.
    Figure Legend Snippet: HeLa cells were co-transfected with GFP-YTHDC1(1–353), Myc-SRSF3, or Myc-SRSF3 deletion mutants. The lysates of co-transfected cells were immunoprecipitated using anti-GFP antibody. Lysates and IP samples were detected by western blotting using anti-GFP or anti-Myc antibodies.

    Techniques Used: Transfection, Immunoprecipitation, Western Blot



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    ythdc1 expression vectors  (Genecopoeia)
    94
    Genecopoeia ythdc1 expression vectors
    Primers used in this study.
    Ythdc1 Expression Vectors, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ythdc1 expression vectors/product/Genecopoeia
    Average 94 stars, based on 1 article reviews
    ythdc1 expression vectors - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier

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    Primers used in this study.

    Journal: Cells

    Article Title: Multiple Phosphorylations of SR Protein SRSF3 and Its Binding to m 6 A Reader YTHDC1 in Human Cells

    doi: 10.3390/cells11091461

    Figure Lengend Snippet: Primers used in this study.

    Article Snippet: SRSF3 or YTHDC1 expression vectors were constructed from the pReceiver vector (Genecopoeia) and PCR amplified SRSF3 and YTHDC1 sequences from extracted Hela mRNA.

    Techniques: Sequencing

    Interaction validation of YTHDC1 with SRSF3. ( a ) YTHDC1 and its fragment structure indicating the position of glutamic acid-rich region (Glu-rich), YT521-B homology (YTH) domain and arginine-rich region (Arg-rich). ( b ) HeLa cells were co-transfected with GFP-SRSF3, Myc-YTHDC1, or their mutants. The lysates of co-transfected cells were immunoprecipitated using anti-Myc antibody. Myc-YTHDC1 interaction with GFP-SRSF3 and its fragments was detected by western blotting using anti-GFP or anti-Myc antibodies (( b ), left ); the result of phos-tag gel is also shown in (( b ), right ). Some nonspecific bands are observed.

    Journal: Cells

    Article Title: Multiple Phosphorylations of SR Protein SRSF3 and Its Binding to m 6 A Reader YTHDC1 in Human Cells

    doi: 10.3390/cells11091461

    Figure Lengend Snippet: Interaction validation of YTHDC1 with SRSF3. ( a ) YTHDC1 and its fragment structure indicating the position of glutamic acid-rich region (Glu-rich), YT521-B homology (YTH) domain and arginine-rich region (Arg-rich). ( b ) HeLa cells were co-transfected with GFP-SRSF3, Myc-YTHDC1, or their mutants. The lysates of co-transfected cells were immunoprecipitated using anti-Myc antibody. Myc-YTHDC1 interaction with GFP-SRSF3 and its fragments was detected by western blotting using anti-GFP or anti-Myc antibodies (( b ), left ); the result of phos-tag gel is also shown in (( b ), right ). Some nonspecific bands are observed.

    Article Snippet: SRSF3 or YTHDC1 expression vectors were constructed from the pReceiver vector (Genecopoeia) and PCR amplified SRSF3 and YTHDC1 sequences from extracted Hela mRNA.

    Techniques: Biomarker Discovery, Transfection, Immunoprecipitation, Western Blot

    Interaction of YTHDC1 fragment with SRSF3. ( a ) YTHDC1 and its 493–727 fragment structure. ( b ) HeLa cells were co-transfected with GFP-SRSF3 wild type and mutants, and Myc-YTHDC1(493–727). The interaction between the C-terminal region of YTHDC1 with GFP-SRSF3 and its fragments was detected by western blotting using anti-GFP or anti-Myc antibodies. Some nonspecific bands are observed.

    Journal: Cells

    Article Title: Multiple Phosphorylations of SR Protein SRSF3 and Its Binding to m 6 A Reader YTHDC1 in Human Cells

    doi: 10.3390/cells11091461

    Figure Lengend Snippet: Interaction of YTHDC1 fragment with SRSF3. ( a ) YTHDC1 and its 493–727 fragment structure. ( b ) HeLa cells were co-transfected with GFP-SRSF3 wild type and mutants, and Myc-YTHDC1(493–727). The interaction between the C-terminal region of YTHDC1 with GFP-SRSF3 and its fragments was detected by western blotting using anti-GFP or anti-Myc antibodies. Some nonspecific bands are observed.

    Article Snippet: SRSF3 or YTHDC1 expression vectors were constructed from the pReceiver vector (Genecopoeia) and PCR amplified SRSF3 and YTHDC1 sequences from extracted Hela mRNA.

    Techniques: Transfection, Western Blot

    ( a ) HeLa cells were co-transfected with Myc-YTHDC1, GFP-SRSF3, or GFP-SRSF3 deletion mutants. The lysates of co-transfected cells were immunoprecipitated using anti-Myc antibodies. Lysates and IP samples were detected by western blotting using anti-GFP or anti-Myc antibodies. Phosphorylation status of immunoprecipitated SRSF3 and mutants was analyzed by separation using phos-tag gel (bottom panel). ( b ) YTHDC1 and its 1–353 fragment structure. ( c ) HeLa cells were co-transfected with GFP-YTHDC1(1–353), Myc-SRSF3, or Myc-SRSF3 deletion mutants. Lysates of co-transfected cells were immunoprecipitated using anti-GFP antibody. Lysates and IP samples were detected using anti-GFP or anti-Myc antibodies. YTHDC1(1–353) shows a clear interaction with SRSF3(1–132).

    Journal: Cells

    Article Title: Multiple Phosphorylations of SR Protein SRSF3 and Its Binding to m 6 A Reader YTHDC1 in Human Cells

    doi: 10.3390/cells11091461

    Figure Lengend Snippet: ( a ) HeLa cells were co-transfected with Myc-YTHDC1, GFP-SRSF3, or GFP-SRSF3 deletion mutants. The lysates of co-transfected cells were immunoprecipitated using anti-Myc antibodies. Lysates and IP samples were detected by western blotting using anti-GFP or anti-Myc antibodies. Phosphorylation status of immunoprecipitated SRSF3 and mutants was analyzed by separation using phos-tag gel (bottom panel). ( b ) YTHDC1 and its 1–353 fragment structure. ( c ) HeLa cells were co-transfected with GFP-YTHDC1(1–353), Myc-SRSF3, or Myc-SRSF3 deletion mutants. Lysates of co-transfected cells were immunoprecipitated using anti-GFP antibody. Lysates and IP samples were detected using anti-GFP or anti-Myc antibodies. YTHDC1(1–353) shows a clear interaction with SRSF3(1–132).

    Article Snippet: SRSF3 or YTHDC1 expression vectors were constructed from the pReceiver vector (Genecopoeia) and PCR amplified SRSF3 and YTHDC1 sequences from extracted Hela mRNA.

    Techniques: Transfection, Immunoprecipitation, Western Blot, Phospho-proteomics

    HeLa cells were co-transfected with GFP-YTHDC1(1–353), Myc-SRSF3, or Myc-SRSF3 deletion mutants. The lysates of co-transfected cells were immunoprecipitated using anti-GFP antibody. Lysates and IP samples were detected by western blotting using anti-GFP or anti-Myc antibodies.

    Journal: Cells

    Article Title: Multiple Phosphorylations of SR Protein SRSF3 and Its Binding to m 6 A Reader YTHDC1 in Human Cells

    doi: 10.3390/cells11091461

    Figure Lengend Snippet: HeLa cells were co-transfected with GFP-YTHDC1(1–353), Myc-SRSF3, or Myc-SRSF3 deletion mutants. The lysates of co-transfected cells were immunoprecipitated using anti-GFP antibody. Lysates and IP samples were detected by western blotting using anti-GFP or anti-Myc antibodies.

    Article Snippet: SRSF3 or YTHDC1 expression vectors were constructed from the pReceiver vector (Genecopoeia) and PCR amplified SRSF3 and YTHDC1 sequences from extracted Hela mRNA.

    Techniques: Transfection, Immunoprecipitation, Western Blot